*Applications may be reviewed on a rolling-basis and this posting could close before the deadline. Click here for information about the selection process.
EPA Office/Lab and Location: A research opportunity is available at the Environmental Protection Agency (EPA), Office of Research and Development (ORD), Center for Computational Toxicology and Exposure (CCTE), Biomolecular & Computational Toxicology Division (BCTD) located in Research Triangle Park, North Carolina.
Research Project: This research project will develop and implement CRISPR methodology to genetically modify cell culture systems to mimic susceptibility factors that may augment the adverse effects of environmental chemical exposure. These data, which will include traditional toxicological and novel high-throughput phenotypic and single cell transcriptomic assessments, will be incorporated into a quantitative framework which will help predict the impact of these susceptibilities to environmental stressors that can help in later risk determinations.
The research participant will collaborate in developing methods for dosing of cell culture, and generating phenotypic data to gather the needed data to structure the computational model. Research activities may include:
- Hands-on participation in experimental research and data interpretation
- Reading and interpreting relevant scientific literature
- Active participation in meetings of the project team
- Preparing reports, presentations, and summaries of the data
- Presenting at professional meetings
- Authoring manuscripts for publication in peer-reviewed journals.
Learning Objectives: Under the guidance of a mentor, the participant will be involved in the following research activities:
- Developing methods to edit DNA/epigenome using CRISPR systems
- Generating, analyzing and integrating phenotypic and transcriptomic data
- Maintaining and exposing cell culture to environmental chemical stressors
- Synthesizing results for publication and presentation
Mentor(s): The mentor for this opportunity is Brian Chorley (firstname.lastname@example.org). If you have questions about the nature of the research please contact the mentor(s).
Anticipated Appointment Start Date: Summer 2021. All start dates are flexible and vary depending on numerous factors. Click here for detailed information about start dates.
Appointment Length: The appointment will initially be for one year and may be renewed up to three or four additional years upon EPA recommendation and subject to availability of funding.
Level of Participation: The appointment is full-time.
Participant Stipend: The participant will receive a monthly stipend commensurate with educational level and experience. Click here for detailed information about full-time stipends.
EPA Security Clearance: Completion of a successful background investigation by the Office of Personnel Management (OPM) is required for an applicant to be on-boarded at EPA.
ORISE Information: This program, administered by ORAU through its contract with the U.S. Department of Energy (DOE) to manage the Oak Ridge Institute for Science and Education (ORISE), was established through an interagency agreement between DOE and EPA. Participants do not become employees of EPA, DOE or the program administrator, and there are no employment-related benefits. Proof of health insurance is required for participation in this program. Health insurance can be obtained through ORISE.
Questions: Please see the FAQ section of our website. After reading, if you have additional questions about the application process please email ORISE.EPA.ORD@orau.org and include the reference code for this opportunity.